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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense

Fig. 2

The GPER agonist, G1 attenuates protein expression of the NLRP3 inflammasome components in the hippocampal CA1 region following GCI. a Immunofluorescent staining of NLRP3 (green) and Iba1 (red), b CD11b (red), and ASC (green), showing the increased intensity of NLRP3 and ASC in the 14-day IR group, as compared to the G1-treated group, and G36 reversed the effect. Co-expression of NLRP3 with Iba1 or ASC with CD11b is indicated as yellow in the merged photomicrographs. c Western blot analysis of NLRP3 and ASC. Quantification was carried out according to the density of blot bands ratio to GAPDH. Data was expressed as means ± SE. GAPDH was used as a loading control. d Duolink analysis showed the interaction (red particles) of NLRP3 with ASC in both the 14-day IR and G36-treated groups compared to the sham or G1 group, DAPI (blue) counterstaining nucleus. #P < 0.05 vs. sham group, ##P < 0.05 vs. 14-day IR group, ###P < 0.05 vs. G1-treated group. n = 4–5, scale bar 50 μm

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Journal of Neuroinflammation

ISSN: 1742-2094