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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense

Fig. 3

The effects of the GPER agonist, G1 upon caspase 1 activation and IL1β production in the hippocampal CA1 region following GCI. ad Western blot analysis for CD11b, a marker of activated microglia, Cle-cas1 (cleaved caspase 1) and cleaved IL1β. Semi-quantitative analysis was carried out according to the band density of target protein ratio to that of loading control (GAPDH). Data was expressed as mean ± SE. #P < 0.05 vs. 14-day IR group, ##P < 0.05 vs. G1-treated group, e Double-immunofluorescent staining for CD11b (red) and cleaved IL1b (green), showing a strong increase in CD11b and cleaved IL1b intensity in the 14-day IR animals compared to the G1-treated group, while G36 reversed the increase. Scale bar 50 μm, magnification 40× n = 4–5

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