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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense

Fig. 5

IL1RA knockdown abolishes the anti-inflammatory effects of GPER activation at 3 days of reperfusion in the hippocampal CA1 region following GCI. a Western blot analysis showed that IL1RA antisense oligodeoxynucleotide (AS) knockdown significantly decreased IL1RA protein expression (n = 4 in each group). Samples for Western blot were from sham, IR, G1 plus IL1RA missense oligodeoxynucleotide (MS), and G1 plus AS. Antibodies of CD11b (n = 4 in sham, IR, G1 groups, and n = 5 in G36 + G1-treated group), NLRP3 (b) (n = 4 in each group), cleaved-IL1β (c) (n = 4 in sham, IR, G1 groups, and n = 5 in G36 + G1-treated group), and loading control GAPDH. #P < 0.05 vs. 3-day IR group, ##P < 0.05 vs. G1 + MS group. Double staining for NLRP3 (green) and CD11b (red) (d) or cleaved-IL1b (green) and CD11b (red) (e) in the indicated groups. × 40 magnification, scale bar 50 μm

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