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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense

Fig. 6

IL1RA knockdown reverses G1 regulation of NF-κB signaling in the hippocampal CA1 region following GCI. a-b Western blot analysis for NF-κB p65 in cytoplasm and nucleus fractions, and GAPDH, H2A as the loading controls of cytoplasm and fraction, respectively. #P < 0.05 vs. sham group, ##P < 0.05 vs. 3-day IR group, and ###P < 0.05 vs. G1 + MS group. c Double straining of NF-κB-p65 (red) and Iba1 (green). d Colocalization analysis of NFkB-P65 and Iba1 by Pearson Correlation Coefficient (PCC). #< 0.05 vs. 3-day IR group, and ##< 0.05 vs. G1 + MS group. × 40 magnification, scale bar 50 μm, n = 4–5

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