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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense

Fig. 7

IL1RA knockdown abolishes the neuroprotective effect of G1 following GCI in the hippocampal CA1 region. a Western blot analysis of anti-apoptotic protein Bcl2, CREB activation, and an executioner of apoptosis, cle-cas3 (cleaved caspase 3). b Semi-quantitative analysis was carried out according to the band density of Bcl2 or cle-cas3 and p-CREB (c) ratio to that of loading control (tubulin). d Representative images of double-immunofluoscence staining for NeuN (red) and p-CREB (green). Data was expressed as mean ± SE. #P < 0.05 vs. 3-day IR group, ##P < 0.05 vs. G1 + MS-treated group. n = 4–5. e–g Representative photomicrographs of NeuN staining (green) and TUNEL analysis (red) in the indicated groups. n = 5, scale bar 50 μm

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Journal of Neuroinflammation

ISSN: 1742-2094