Fig. 1

Injury model and analysis methodology. a A rostral view of an extracted pig brain. The arrow demonstrates the coronal plane rotation. b A sagittal view of an extracted pig brain. The right arrow shows the location of the anterior hippocampal tissue (c; scale bar = 1 cm), while the left arrow shows the location of the posterior hippocampal tissue (d). e All tissue was imaged on a confocal microscope for Hoechst (blue), synapsin (green), MAP2 (red), and Iba-1 (purple). After imaging, each mossy cell was outlined to obtain the area of each cell and a 30-μm radius circle was created around the epicenter of each mossy cell (scale bar = 20 μm). f Highlights the extent of positive pixels detected by the Nikon Elements software, which were analyzed for the current study. Synapsin-positive pixels are shown in neon green and Iba-1-positive pixels are shown in white