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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Mossy cell hypertrophy and synaptic changes in the hilus following mild diffuse traumatic brain injury in pigs

Fig. 4

Microglia density proximal to mossy cells changes following mild TBI. To examine the migration of microglia after injury, we counted Iba-1-positive cells within the ROI around each mossy cell from sham, 7 DPI, and 30 DPI specimens in the anterior and posterior hippocampal hilus. Additionally, we also counted the Iba-1-positive cells that directly contacted the soma of each identified mossy cell. Representative images of anterior hilar mossy cells from sham (a), 7 DPI (d), and 30 DPI (g) specimens are shown, while n, q, and t correspond to the posterior hilar mossy cells of those animals (scale bar = 25 μm). Representative images of microglia activity in anterior hippocampal hilus from sham (b), 7 DPI (e), and 30 DPI (h) specimens are shown, while o, r, and u correspond to the posterior hilar microglia activity of those animals. Merged images of MAP2 and Iba-1 in anterior and posterior hilus are shown in c, f, and i and p, s, and v respectively. j There was a non-significant decrease in microglia density in anterior hilus at 7 DPI (p = 0.1377) and 30 DPI (p = 0.2258) compared to sham. Data represent mean +/− SEM. l A histogram of the number of Hoechst-positive microglia per mossy cell within this ROI in anterior hilus indicates a leftward shift of the data distribution for 7 DPI and 30 DPI compared to sham. k There was a nonsignificant decrease in the number of microglia on anterior hilar mossy cell somata at 7 DPI (p = 0.0889) and 30 DPI (p = 0.0794). m A histogram of the number of microglia contacting anterior hilar mossy cell somata demonstrates a leftward shift of the data distribution at 7 DPI and 30 DPI compared to sham. w There was no change in microglia density within the ROI at 7 DPI (p = 0.9573) and 30 DPI (p = 0.7254) compared to sham in posterior hilus. x There was no significant change in the number of microglia on posterior hilar mossy cell somata at 7 DPI (p = 0.3446) and 30 DPI (p = 0.5170). y, z Histograms of microglia density within the ROI and on mossy cell somata in posterior hilus did not reveal additional data trends

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