Fig. 5

MiR-216a-5p is upregulated in HExos and transferred to BV2 and primary microglia by exosomes. a Heat map of the 80 upregulated and 46 downregulated miRNAs with a ≥ 1.5-fold difference between Exos and HExos derived from BMSCs. b Comparison of the top five elevated miRNAs including miR-216a-5p, miR-99b-5p, miR-301a, miR-126 and miR-210-3p between Exos and HExos using qRT-PCR. c miR-216a-5p overexpression and knockdown in BMSCs and the efficiency confirmed using qRT-PCR. d The relative expression level of miR-216a-5p in exosomes derived from hypoxic BMCSs transfected with miR^OE, miR-NC^OE, miR^KD and miR-NC^KD. e Expression level of miR-216a-5p in target BV2 microglia and primary microglia after administering miR-NC^KD-HExos, miR^KD-HExos, miR-NC^OE-HExos and miR^OE-HExos. f Representative images of FAM-labeled exosomal miR-216a-5p internalized by BV2 microglia after administration of miR-NC^KD-HExos, miR^KD-HExos, miR-NC^OE-HExos and miR^OE-HExos. ^*P < 0.05