Skip to main content
Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Exosome-shuttled miR-216a-5p from hypoxic preconditioned mesenchymal stem cells repair traumatic spinal cord injury by shifting microglial M1/M2 polarization

Fig. 8

Exosomal miR-216a-5p regulates TLR4 by directly targeting the 3′-UTR. a and b Luciferase reporter assay was performed to confirm that TLR4 is the target gene of miR-216a-5p. c Immunoprecipitation of the Ago2/RISC (RNA-induced silencing complex) using the Pan-Ago2 antibody in BV2 microglia overexpressing miR-NC or miR-216a-5p. IgG was used as a negative control and β-actin was used as an internal control. d The mRNA expression level of TLR4 in microglia after miR-216a-5p overexpression and knockdown by qRT-PCR. e The protein expression level of TLR4 in microglia after miR-216a-5p overexpression and knockdown by western blot analysis. *P < 0.05

Back to article page