Fig. 6
CLEC14A KO mice exhibited glial activation after ischemia-reperfusion. a Picture of TTC stained coronal brain section indicating immunofluorescence staining regions. The square fields represent the observed regions of GFAP (red) and Iba1 (green). b Immunofluorescence staining of GFAP (red) and Iba1 (green) in the ischemic cortex of the WT and CLEC14A KO groups after ischemia-reperfusion. Increased expression of GFAP and Iba1 was present in the CLEC14A-KO group, compared with the WT group. n = 6 per group. Scale bars: 100 μm. c, d Glial activation was quantified based on the percentage of the GFAP-positive area and Iba1 positive cells per mm2. *P < 0.05, **P < 0.01, and ***P < 0.001, paired comparisons. The results are mean values and the error bars represent the mean ± SD