Table 1 Primer sequences used for RT-PCR
Primer | Direction | Sequence |
|---|---|---|
IL-6(M) | Fwd | 5′-TACCACTTCACAAGTCGGAGGC-3’ |
Rev | 5′-CTGCAAGTGCATCATCGTTGTTC-3’ | |
IL-1b(M) | Fwd | 5′-TGGACCTTCCAGGATGAGGACA-3’ |
Rev | 5′-GTTCATCTCGGAGCCTGTAGTG-3’ | |
TNF-a(M) | Fwd | 5′-GACGTGGAACTGGCAGAAGA-3’ |
Rev | 5′-CCGCCTGGAGTTCTGGAA-3’ | |
MCP-1(M) | Fwd | 5′-CACTCACCTGCTGCTACTCA-3’ |
Rev | 5′-CTTCTTGGGGTCAGCACAGA-3’ | |
GAPDH(M) | Fwd | 5′-CATCACTGCCACCCAGAAGACTG-3’ |
Rev | 5′-ATGCCAGTGAGCTTCCCGTTCAG-3’ |