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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: A novel role of NLRP3-generated IL-1β in the acute-chronic transition of peripheral lipopolysaccharide-elicited neuroinflammation: implications for sepsis-associated neurodegeneration

Fig. 3

High dose LPS injection produces long-lasting microglial activation in WT mice, but not in NLRP3−/− or IL-1R1−/− mice. Following a single injection of LPS (5 mg/kg; ip) or saline vehicle, mice were perfused 6 h, 1 week, 4 and 10 months thereafter for nigral microglial Iba-1 or CD-11b immunostaining (n = 3/group for each time point). Representative images at each time point were shown (ad). Scale bar = 300 μm. Results are expressed as folds of time-matched vehicle control. Histograms represent degree of microglial activation quantified by measuring the density of Iba-1 or CD-11b staining with ImageJ. ***p < 0.001, ****p < 0.0001, and NS compared to respective saline vehicle group. Two-way ANOVA followed by Bonferroni post hoc multiple comparison test

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