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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Glial remodeling enhances short-term memory performance in Wistar rats

Fig. 7

Microglial repopulation is associated with increased astrocyte activity. Astrocyte profiles at 7 days after diphtheria toxin injection to ablate microglia in Cx3cr1-Dtr rats compared to wild-types (wt). a Hippocampal expression of Gfap. b hippocampal astrocyte density (hilus: t(11) = 2.67, p = 0.022. The p values in the CA3, subgranular/granular and molecular regions for the comparison between the wt and Cx3cr1-Dtr groups were 0.073, 0.060, and 0.088 respectively). c Representative photomicrographs of GFAP. d Astrocyte phagocytosis of polystyrene beads in the CA1, CA3, and Hilus regions (t(6) = 2.61, p = 0.040). e Representative photomicrographs of GFAP-positive cells with phagocytosed microbeads (block arrows). f Example of an astrocyte and its reconstruction showing co-localization of the beads inside the cell (block arrow) and in the process of centering the cell (open arrow). Internalization was verified by imaging the region as a z-stack, transforming the z-stack into a 3D image and visually ensuring that the maximum fluorescence intensity started from within the cell. g Astrocyte profiles at 2 days after diphtheria toxin injection (CA1: t(9) = 3.51, p = 0.007; CA3: t(9) = 5.52, p = 0.007; hilus: t(10) = 2.62, p = 0.025; n = 6 per group). *p < 0.05; Student’s unpaired t tests. Data are expressed as mean ± SEM. Scale bars C = 50 μm; E = 20 μm; F = 10 μm

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