Fig. 3

CXCR2⁺ neutrophils are not required for CNS infiltration of immune cells. Numbers of total mononuclear cells, adaptive immune cells (B cells: CD45⁺CD19⁺; CD4⁺T cells: CD45⁺CD3⁺CD4⁺; CD8⁺T cells: CD45⁺CD3⁺CD8⁺), innate immune cells (neutrophils: CD45⁺+Ly6G^high; dendritic cells: CD45⁺ CD11c⁺; macrophages: CD45⁺CD11b⁺Ly6G⁻CD11c⁻), and CNS resident glia (microglia: CD45^lowCD11b⁺) in the a brain and b spinal cord of control or Cxcr2 cKO mice. Numbers of total mononuclear cells, adaptive immune cells (B cells: CD19⁺; CD4⁺T cells: CD3⁺CD4⁺; CD8⁺T cells: CD3⁺CD8⁺), and innate immune cells (neutrophils: CD11b⁺Ly6G^high; dendritic cells: CD11c⁺; macrophages: CD11b⁺ Ly6G⁻CD11c⁻) in the c spleen and d lymph nodes of control or Cxcr2 cKO mice. Control EAE, n = 5; Cxcr2 cKO EAE, n = 7. *p < 0.05, two-tailed unpaired Student’s t test