Fig. 5

CXCR2 in neutrophils is required for neutrophil-induced neuronal damage in vitro. a Representative images of N2a neurons co-cultured with neutrophils isolated from control naïve mice, control EAE mice, or Cxcr2 cKO EAE mice, showing 7-aad neuronal cell death fluorescent signals. b Quantification of 7-aad⁺ neurons 18-h post-co-culture with ex vivo neutrophils. A total of 15,007 N2a cells were included in our analyses of 7-aad⁺N2a cells (control naïve, 2965 N2a cells examined; control EAE, 4259 N2a cells examined; Cxcr2 cKO EAE, 7852 N2a cells examined). *p < 0.05, two-tailed unpaired Student’s t test