Fig. 12

Effects of ligature-induced periodontitis on level of Aβ. ELISA was performed to detect the total level of Aβ and the Aβ ratio (Aβ1-40/Aβ1-42) in the plasma and cortex: a total level of Aβ in the plasma (n = 6, F = 8.239, p = 0.0009), b total level of Aβ in the cortex (n = 6, F = 8.778, p = 0.0006), c the Aβ ratio in the plasma (n = 4, F = 8.031, p = 0.0033), d the Aβ ratio in the cortex (n = 4, F = 5.304, p = 0.0147). Immunohistochemistry was used to conduct histopathological analysis of brain sections. Cytoplasmic yellow/brown cytoplasm was observed in both the hippocampus and cortex of the CP group. Quantification of Aβ1-42 levels was shown: e Aβ1-42 positive cells in the hippocampus (n = 3, F = 29.83, p = 0.0001), f Aβ1-42-positive cells in the cortex (n = 3, F = 24.53, p = 0.0002), (100× and 400×, bar = 200 μm and 50 μm, respectively). (n = 4–6 per group for ELISA and n = 3 per group for IHC analysis, one-way ANOVA, *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the control group; #p < 0.05, ##p < 0.01, and ###p < 0.001 compared to the CP group)