Fig. 2

Effects of periodontal ligature on the periodontal tissue. a Images captured by micro-CT scanning of the molar region. Bone density analysis of the alveolar bone: b BV/TV (n = 4, F = 7.062, p = 0.0054), c BS/BV (n = 4, F = 5.488, p = 0.00131), and d BMD (n = 4, F = 7.323, p = 0.0048). After decalcification, hematoxylin-eosin (HE) staining assay was used to detect the inflammation response in maxillae of rats (e). Microscope images were at a magnification of ×100 and ×400 (bar = 200 μm and 50 μm, respectively). Quantified analysis conducted by ImageJ: f staining area (n = 3, F = 33.19, p < 0.0001) and g number of cells/area (n = 3, F = 11.72, p = 0.0027). (n = 3–4 per group, one-way ANOVA, **p < 0.01 and ***p < 0.001 compared to the control group; #p < 0.05 and ##p < 0.01 compared to the CP group)