Fig. 2
From: Interactions of neuroimmune signaling and glutamate plasticity in addiction
High-resolution 3-D image of an isolated Iba-1-positive microglia with orthogonal views. a Iba-1 labeling and modern confocal microscopy can be reliable used to label and image microglial syncytia (white). Within these syncytia, individual microglia (green) can be digitally isolated from neighboring cells and subsequently analyzed. b Here a space filling 3D render of the isolated microglia is shown (grey). This type of digital analyses can be used to obtain data for general physical parameters (surface area and volume). c Here the microglial cell of interest is shown (green) overlaid with a skeletonization (white). This type of analysis can be used to obtain structural characteristics (Sholl intersections, branch number, and branch order). Hashed boxes depict locations of inset panels. Scale bar depicts 10 μm