Fig. 1

Mice with a FoxP3-specific deletion of Blimp1 develop severe EAE associated with highly activated T and myeloid cells. a EAE was induced with MOG_35-55/CFA in FoxP3^YFP-Cre (WT) or Prdm1^fl/flFoxP3^YFP-Cre (KO) mice. EAE disease scores are shown. b Frequency of GM-CSF⁺ and IL-17A⁺ CD4⁺FoxP3⁻ Teff from EAE mice as in a. SP, spleen; SC, spinal cord. c Expression of GM-CSF in brain microglia (CD45^medCD11b⁺) (left) and frequency of GM-CSF⁺ microglia (right). d CD45^hiCD11b^hi subsets from the brain were gated based on Gr-1 levels. Right, ratios of Gr-1⁺ (Gr-1^hi + Gr-1^med) to Gr-1^lo. e iNOS expression in Gr-1-expressing cells from D. Bottom, quantitation of mean fluorescence intensity (MFI) of iNOS. Data represent one of at least three experiments (a–e: n = 5/group). In d, mice were pooled from two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (a, two-way ANOVA post hoc Sidak’s multiple comparisons test; b–e, unpaired two-tailed Student’s t test). Bars, mean ± SEM