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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Dysregulated follicular regulatory T cells and antibody responses exacerbate experimental autoimmune encephalomyelitis

Fig. 1

Mice with a FoxP3-specific deletion of Blimp1 develop severe EAE associated with highly activated T and myeloid cells. a EAE was induced with MOG35-55/CFA in FoxP3YFP-Cre (WT) or Prdm1fl/flFoxP3YFP-Cre (KO) mice. EAE disease scores are shown. b Frequency of GM-CSF+ and IL-17A+ CD4+FoxP3 Teff from EAE mice as in a. SP, spleen; SC, spinal cord. c Expression of GM-CSF in brain microglia (CD45medCD11b+) (left) and frequency of GM-CSF+ microglia (right). d CD45hiCD11bhi subsets from the brain were gated based on Gr-1 levels. Right, ratios of Gr-1+ (Gr-1hi + Gr-1med) to Gr-1lo. e iNOS expression in Gr-1-expressing cells from D. Bottom, quantitation of mean fluorescence intensity (MFI) of iNOS. Data represent one of at least three experiments (ae: n = 5/group). In d, mice were pooled from two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (a, two-way ANOVA post hoc Sidak’s multiple comparisons test; be, unpaired two-tailed Student’s t test). Bars, mean ± SEM

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