Fig. 3

Blimp1-deficient Tregs display impaired CNS Treg features and are insufficient to regulate CNS CD11b⁺ cells. a Expression of indicated proteins in FoxP3⁺ Tregs from the spleen (SP), spinal cord (SC), and brain (BR) of mice at d20 post-EAE induction, as in Fig. 1a. b MFI of each protein expressed in SC Tregs in a. c Splenic YFP⁺(FoxP3⁺) Tregs and CNS CD45⁺CD11b⁺ cells were sorted from MOG-immune mice and were co-cultured in triplicates with or without IL-33 for 40 h. Histogram overlays of Arg-1 and iNOS in CD11b⁺ cells. d Frequencies of Arg-1⁺ or iNOS⁺ CD11b⁺ cells in c. e Histogram overlays of GM-CSF in Tregs in C. Bottom, frequencies of GM-CSF⁺ Tregs. WT, FoxP3^YFP-Cre; KO, Prdm1^fl/flFoxP3^YFP-Cre. Data represent one of two experiments (b n = 5/group). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (b, unpaired two-tailed Student’s t test; d, e, one-way ANOVA post hoc Tukey’s multiple comparisons test). Bars, mean ± SEM