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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Dysregulated follicular regulatory T cells and antibody responses exacerbate experimental autoimmune encephalomyelitis

Fig. 5

Increased activation of CNS CD11b+ cells after culture with serum from Prdm1fl/flFoxP3YFP-Cre EAE mice. a Sera from WT or KO EAE mice were added into the culture of CNS CD11b+ cells for 48 h. Flow cytometry of histogram overlays of CD68, MHCII, and TNFα in CD11b+ cells. b MFI of CD68 and MHCII, and frequencies of TNFα+ CD11b+ cells (as in a) cultured with or without sera in triplicates of each condition. Groups of cells pre-treated with anti-IgE or anti-IgG Ab were included. Ctrl, no mouse sera added; WT, sera from FoxP3YFP-Cre EAE mice; KO, sera from Prdm1fl/flFoxP3YFP-Cre EAE mice. Data represent one of two experiments. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (b, one-way ANOVA post hoc Tukey’s multiple comparisons test). Bars, mean ± SEM

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