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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats

Fig. 1

Expression levels of α-MSH, MC1R, and Nurr1 post-HI. a Representative pictures of Western blot data. b Western blot data showed that the expression levels of α-MSH significantly increased at 12 h post-HI, and peaked at 24 h. c, d The endogenous expression levels of MC1R and Nurr1 increased over time and peaked at 48 h post-HI. Data were represented as mean ± SD. Statistical differences between groups were analyzed using one-way ANOVA, followed by Tukey’s post hoc test (*p < 0.05 versus sham, #p < 0.05 versus 6 h HI, @p < 0.05 versus 24 h HI, &p < 0.05 versus 48 h HI; n = 4 per group). e Representative microphotographs of double immunofluorescence of α-MSH (green) with microglia (Iba-1, red), astrocyte (GFAP, red), and neuron (NeuN, red) in the peri-infarcted area at 24 h post-HI. DAPI was stained blue. Merged images showed that α-MSH was colocalized with microglia, astrocytes, and neurons. n = 2 per group. Scale bar = 100 μm

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