Fig. 3

Immunofluorescence staining of MC1R and Nurr1 with microglia in the brain at 48 h post-HI. Immunofluorescence staining showed an increase expression of MC1R (a) and Nurr1 (b) on microglia in the vehicle group when compared with the sham group, and further increased expression of MC1R (a) and Nurr1 (b) in the BMS-470359 treatment group. Merge showed the colocalization of MC1R and Nurr1 on microglia. Microglia were stained red. MC1R and Nurr1 were stained green. DAPI was stained blue. n = 2 per group. Scale bar = 100 μm