Fig. 6

MC1R activation increased the expression of CD206 at 48 h post-HI. Representative microphotographs of immunofluorescence staining and quantification of CD206 with microglia (CD11 b/c) at 48 h post-HI. a, b The number of CD206⁺ CD11 b/c⁺ cells significantly increased in the vehicle group compared to the sham group. Activation of MC1R with BMS-470539 further increased the number of CD206⁺ CD11 b/c⁺ cells compared to the vehicle group, while knockout CRISPR interventions significantly reversed these effects. CD206 was green. CD11 b/c was red. Blue was for DAPI. Scale bar = 100 μm. Data was represented as mean ± SD. The one-way ANOVA was followed by Tukey’s post hoc test (*p < 0.05 versus sham; ^#p < 0.05 versus HI + vehicle; ^@p < 0.05 HI + BMS-470539 or HI + BMS-470539 + control CRISPR; n = 6 per group)