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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Metabotropic glutamate receptor 5 inhibits α-synuclein-induced microglia inflammation to protect from neurotoxicity in Parkinson’s disease

Fig. 5

Activation of mGluR5 partially protected neurons from neurotoxicity caused by α-syn-induced microglia activation. ad Primary microglia were infected with LV-NC or LV-α-syn followed by MTEP (100 μM), CHPG (150 μM), or urate (200 μM) treatment for 24 h, and mGluR5 protein expression was detected by western blotting (a, left). Quantification of mGluR5 level was normalized to β-tubulin (a, right). NO (b), PGE2 (c), and TNF-α (d) levels in the culture supernatants were measured by ELISA. Cells infected with LV-NC without drugs administration served as control (ad). e The purity of primary neurons and primary microglia were evaluated by immunofluorescence detection of NeuN (top, green, scale bar, 50 μm) and Iba-1 (bottom, red, scale bar, 25 μm), respectively. Cell nuclei were stained with DAPI. f, g Primary neurons were incubated for 24 h with conditioned medium from cultures of rat primary microglia infected with LV-α-syn or LV-NC followed by MTEP (100 μM), CHPG (150 μM), or urate (200 μM) treatment. Cell viability was measured with the MTS assay (f) or CCK-8 assay (g). Medium from cultures of microglia infected with LV-NC served as control (f, g). Data shown in this figure represent mean ± SEM (n ≥ 3). The statistical significance was determined by one-way ANOVA followed by Dunnett’s test. ***p < 0.001 versus control (ctr); #p < 0.05, ##p < 0.01, and ###p < 0.001 versus the LV-α-synuclein (α-syn) infection; ns, not significant

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