Fig. 3
Immunofluorescence staining and flow cytometric analysis of CD4+ T cell subsets within the retina after GW559090 treatment. a There were both Th1 (CD4+Tbet+) and Th17 (CD4+RORγt+) cells in the vitreous and inner retinal layers in Veh group. b In GW10-treated group, there were decreased numbers of CD4+ T cells infiltrating the retina which were mainly Th1 cells. c Counting the CD4+ T cell subsets within the vitreous revealed that Th17 cells were mostly suppressed by 10 mg/ml GW559090 treatment. d Representative retinal flow cytometric figures for CD4+ T cells. e Identification of CD4+ T cell subsets in the retina by expression of IFNγ or IL-17A. f Representative retinal flow cytometric figures for the distribution of IFNγ or IL-17A within CD4+FoxP3- live cells. g Figures represent the results of flow cytometric analysis for CD4+ T cell subsets within the dLNs. h, i VLA-4 expression in CD4+ T cell subsets within the retina (h) and dLNs (i). j, k Mean fluorescence intensity (MFI) of VLA-4 expression in Th1 and Th17 cells in eye (j) and dLNs (k). Means ± SD. n = 5 in c and n = 3 in e and g–k in each treatment group. Eye data were from single eyes in individual mice. Data were from one experiment which is representative of three separate experiments that were performed. *P < 0.05; **P < 0.01; ***P < 0.001 compared to Veh group, using two-tailed, unpaired Student’s t test