Fig. 4

Localization and effect of GW559090 treatment on myeloid cells. a There were more myeloid cells (CD4^-Iba-1⁺ cells) present within the inner retinal (R) layers in Veh group (× 400). b There were reduced levels of CD4⁺ T cells observed within the vitreous (V) and only very limited numbers of myeloid cells in the eyes of GW₁₀ group (× 400). c Representative flow cytometry figure depicting CD11b⁺CD11c^- myeloid population and CD11b⁺CD11c⁺ dendritic cells (DC) from CD45⁺ cell gates in single live cells. d Level of DCs in each treatment group illustrating a decrease of DCs in the eyes treated with Dex and GW₁₀ compared to the control. e Representative flow cytometry figures illustrating inflammatory monocytes/macrophages (Ly6C⁺) and resident monocytes/macrophages (Ly6C^-) from CD45⁺CD11b⁺CD11C^-CD64⁺L6G^- single live cell gate. f Comparing percentages of inflammatory and resident monocytes/macrophages between treatment groups (d, f). n = 3 data points per group from pooled two eyes for flow cytometric analysis. Data were from a single experiment which is representative of three experiments that were performed. Mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 using a two-tailed unpaired Student’s t test