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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Peripherally misfolded proteins exacerbate ischemic stroke-induced neuroinflammation and brain injury

Fig. 6

The Prion-like propagation of CryABR120G protein in cell cultures. a Protein aggregates in primary mixed neuron-glia cultures detected with a Proteostat aggresome detection kit following incubation with the exosomes (Exo) isolated from Ntg or CryABR120G mice. Scale bar, 50 μm. b Quantitative analysis of (a). c Cultured neurons treated with CryABR120G exosomes showed a reduced ATP level. d Schematic illustration of cell culture assay to determine the prion-like property of CryABR120G protein. e Immunocytochemical staining of transfected myc-flag-tagged wild-type mouse CyAB in neural cells following transduction of CryABR120G aggregate seeds. Scale bar, 20 μm. f Quantitative analysis of (e). g Hypothetical model explaining the prion-like propagation of misfolded CryABR120G protein from the heart to brain. Exosomes containing CryABR120G protein can be transported from the heart to brain through blood circulation. In the brain, CryABR120G protein propagates in a prion-like phenomenon, and causes increased neuroinflammation and abnormal brain function. Data are shown as mean ± SD; n = 6 for (c) and n = 3 for (b), (f). *p < 0.05, **p < 0.01

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