Fig. 3

Effect of pMCAO on the frequency of CD4+ CD25+ FoxP3+ cells in blood and secondary lymphoid tissue of young and aged mice (t = 7 days). Young mice are shown in red, aged mice are shown in blue. Sham mice only received initial incision and exposure of the skull. Naïve mice were age-matched mice who did not undergo any surgery. Two-sided, independent-samples t tests investigated differences between two groups (* = p < 0.05, ** = p < 0.01, *** = p < 0.001. Two-way analysis of variance (ANOVA) tests with post hoc Tukey’s multiple comparisons were performed to investigate differences between three or more groups (# = p < 0.05, ## = p < 0.01, ### = p < 0.001). The number of mice per group is shown in parentheses on x-axis. Box-and-whisker plots exhibit 10–90 percentiles