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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Microglia as target for anti-inflammatory approaches to prevent secondary brain injury after subarachnoid hemorrhage (SAH)

Fig. 2

Microglia accumulation showing cerebral spreading inflammation after SAH (day 14). a Representative immunofluorescence staining of microglia (Iba1) within the brain parenchyma. While in Sham animals only minimal microglia-accumulation was seen (11.157 ± 1.538 cells/HPF, upper left), a significant increase was detected after SAH (32.79 ± 4.77 cells/HPF, upper right, p < 0.0001). LPS preconditioning led to significantly diminished microglia cell numbers in comparison to SAH without treatment, but still showed more microglia accumulation in comparison to sham animals (17.43 ± 2.42 cells/HPF, lower left, p < 0.0001 vs. SAH, p < 0.01 vs. Sham), while in PLX3397-treated animals nearly sham-levels were reached (11.16 ± 1.54 cells/HPF, lower right, p < 0.0001 vs. SAH without treatment). Scale bar = 100 μm. b Mean cell counts ± standard deviations displayed in bar diagram showing the significant differences in microglia accumulation between the four treatment groups. n = 5 per group, ANOVA: **p < 0.01; ****p < 0.0001

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