Fig. 2

Expression of TGR5 after middle cerebral artery occlusion (MCAO). a, b Double immunofluorescence staining and relative fluorescent intensity of TGR5 (red) in GFAP (green) in the penumbra following MCAO. n = 4 per group, *P < 0.05 vs. Sham. Scale bar, 50 μm. c, d Flow cytometry showing that TGR5 accumulates to higher levels in CD11b⁺CD45^intermediate microglia and CD11b⁺CD45^high macrophages at 24 h after MCAO. n = 6 for each group, *P < 0.05 vs. Sham. GFAP, glial fibrillary acidic protein