Fig. 6

TGR5 interacts with Pellino3 after middle cerebral artery occlusion (MCAO). a Double immunofluorescence staining showed that co-localization of TGR5 (red) and Pellino3 (green) is increased in the penumbra 24 h after MCAO. n = 4 per group. b Representative co-IP bands showed that interactions of TGR5 with Pellino3 occurred at 24 h after MCAO. Protein bands of TGR5 and Pellino3 in total protein solution were detected by Western blot and quantitative analysis are reported in right panel. n = 6 for each group. *P < 0.05 vs. Sham, ^#P < 0.05 vs. MCAO + vehicle. Bars represent mean ± SD