Fig. 1

Schematic diagram of the experimental procedure. a The mice received behavior training at day − 1 and behavioral tests on days 1, 3, and 7 after anesthesia/surgery. Anesthesia/surgery (A/S) was performed on day 0. Hippocampal tissues were harvested at the end of the behavioral tests on days 1, 3, and 7. Western blotting analysis of SIRT3 expression was performed on days 1, 3, and 7. The levels of SOD, MDA, TNF-α, IL-1β, and IL-6 were measured on days 1 and 3. Immunofluorescence and western blotting analysis for Iba1 were performed on day 1. Golgi staining and LTP of synaptic function were measured on postoperative day 7. b AAV-SIRT3/VEH virus was microinjected into the CA1 region of the hippocampus 28 days before anesthesia/surgery. The mice received behavioral training at day − 1 and behavioral tests on days 1, 3, and 7 after anesthesia/surgery. Western blotting analysis of SIRT3 expression was performed on days 1, 3, and 7. The levels of SOD, MDA, TNF-α, IL-1β, and IL-6 were measured on days 1 and 3. Immunofluorescence and western blotting analysis for Iba1 were performed on day 1. Golgi staining and LTP of synaptic function were measured on postoperative day 7