Fig. 6

Overexpression of SIRT3 in CA1 region of aged mice. a The location for viral microinjection with a rAAV-CMV-SIRT3-IRES2-mNeonGreen vector (AAV-SIRT3) or a control rAAV-CMV-MCS-IRES2-mNeonGreen vehicle (AAV-VEH). b Fluorescence images showing efficient expression of AAV-SIRT3 vector in CA1 region. Representative images of Western blots showing that AAV-SIRT3 injection increased SIRT3 expression in CA1 cells (c–e). Quantification of SIRT3 expression among the 4 groups (f–h). Representative images of Western blots and quantification of SIRT1 expression among the 4 groups (i–n). Analysis of SOD and MDA levels in the hippocampal CA1 region on postoperative days 1 and 3 (o, p). The data are presented as the means ± standard error of the mean for each group (n = 6). *p < 0.05, **p < 0.01 compared with the C + VEH group, ^#p < 0.05 compared with the A/S + VEH group