Fig. 4

PF543 attenuates neuroinflammation in vitro. HAPI cells were pretreated with 10 nM and 100 nM PF543 for 12 h, followed by LPS treatment for 24 h. a Representative Western blots and quantitative data for iNOS, COX-2, TNF-a, IL-6, and GAPDH expression in each group of microglia. PF543 suppressed proinflammatory mediator release and prevented M1 microglia polarization in a concentration-dependent manner. b Immunofluorescence staining for COX-2 in each of group microglia (scale bar: 100 μm). c HAPI cells were treated with different concentrations of PF543 for 48 h and then the cell viability was evaluated by CCK-8 assay. n = 3 independent experiments. *P < 0.05 and **P < 0.01