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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Platelets and lymphocytes drive progressive penumbral tissue loss during middle cerebral artery occlusion in mice

Fig. 2

Neuronal apoptosis is reduced with GPIb treatment. a Schematic view of brain regions of interest (ROI) analyzed to quantify TUNEL+ cells/neurons and representative immunocytologic stainings. b Quantification of neurons stained with NeuN (Alexa488, green) and subjected to TUNEL assay (TMR red, red) in the ipsilateral hemisphere 2, 3, and 4 h after MCAO or after 2 h of MCAO with additional 6 h of reperfusion overall (ROI 1-5) and c divided in cortex (ROI 1 and 2: neocortex) and striatum (ROI 4 and 5: subcortex) stained with NeuN (Alexa488, green) and subjected to TUNEL assay (TMR red, red) in the ipsilateral hemisphere 2 and 3 h after MCAO in mice treated with rat IgG Fab (Ctrl Fab) or p0p/B Fab (a-GPIb Fab) using × 20 objective. Scale bar 100 μm (n = 16–20 slides). *P< 0.05, **P< 0.01 between the indicated groups, 2-tailed Student t test or, in the case of nonparametric functional outcome, the Wilcoxon–Mann–Whitney U-test was applied

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