Fig. 4

T-cells are required to promote infarct growth during MCAO. Representative images of coronal sections stained with TTC 2, 3, and 4 h after MCAO or after 2 h of MCAO with additional 6 h of reperfusion in Rag1^−/− or WT mice and representative images of coronal sections stained with TTC 4 h after MCAO in Rag1^−/− mice with adoptively transferred T-cells (AT T-cells). Infarcted areas are shown in white. Planimetric analysis was used to quantify the infarct volume. Results are presented as box plots (n = 5–10). *P< 0.05, and ***P< 0.001 between the indicated groups, 2-tailed Student t test or, in the case of nonparametric functional outcome, the Wilcoxon–Mann–Whitney U-test was applied. Nonparametric functional outcome between 3 groups were compared by Kruskal–Wallis test with post hoc Dunn’s multiple comparisons test. b.d., beyond detection level