Fig. 2
Intracellular dialysis limits the Ca2+-induced potentiation of the whole-cell transmembrane currents recorded from human microglia. Histogram representing the mean whole-cell current densities recorded from human microglial cells in unstimulated conditions or in high [Ca2+]i conditions, as indicated, with or without internal dialysis (white or hatched bars, respectively). High [Ca2+]i condition was obtained by using a controlled intracellular solution containing 1 μM free Ca2+. Black circles represent individual cells. High [Ca2+]i values significantly higher than respective low [Ca2+]i values. ANOVA, *p=0.023; **p<0.001. Number of cells, from left to right, 4 (patient #12), 13 (6 cells from #3, 5 from #9, and 2 from #12), 51 (9 cells from patient #1, 17 from #2, 6 from #5, and 19 from #13), 13 (6 cells from #3, 5 from #9, and 2 from #12), respectively