Fig. 7

Effects of antidepressants on A1 and A2 phenotype polarization of astrocytes. Cells were pretreated with or without 10 μM of paroxetine, fluoxetine, sertraline, citalopram, fluvoxamine, or venlafaxine for 30 min followed by stimulation with CytoM for 24 h. A Representative images of C3 immunostaining. B Representative images of S100A10 immunostaining. C Quantification of C3 levels (A) and S100A10 levels (B). Values were expressed relative to the one stimulated with CytoM alone, which was set as 100. Data are means ± SE, n = 3. Statistical comparisons were performed using one-way ANOVA. Different letters indicate p < 0.05. Green, GFAP; red, C3 or S100A10; blue, nuclei; bar size, 50 μm. C3, complement component 3, indicating A1 type of astrocytes; GFAP, glial fibrillary acidic protein; S100A10, S100 calcium-binding protein-A10, indicating A2 type of astrocytes