Table 2 Sequences of the primers used for qPCR validation of RNA-Seq data
Gene name | Primer sequence (5′ to 3′) | Amplicon size (bp) |
|---|---|---|
Beta-actin | Forward: TGTCACCAACTGGGACGATA | 165 |
Reverse: GGGGTGTTGAAGGTCTCAAA | ||
Cxcl13 | Forward: CGACTTTGAAAGGTTGCTTGTA | 219 |
Reverse: ACACTGGATGAATAGGAAACGT | ||
Ccl3 | Forward: AGAGGCAGCGAGTACCAGTCC | 184 |
Reverse: CATAGGAGAAGCAGCAGGCAGTC | ||
Cxcl11 | Forward: GCTGTGACAAAGTTGAAGTGAT | 102 |
Reverse: GTATTGTCTGCATTATGAGGCG | ||
Cd68 | Forward: GCTGTGACAAAGTTGAAGTGAT | 200 |
Reverse: GGCTGGTAGGTTGATTGTCGTCTC | ||
Mettl14 | Forward: TGAGAGGCTTAGACCGAAGTCACC | 104 |
Reverse: GACCACGGCCAGCAGATGTTC | ||
Pcdh18 | Forward: CCAGAGTCACAACAGTCACCAGTC | 109 |
Reverse: CTCAACAGCAGGAGTGGCATGG | ||
Nap1l4 | Forward: GGCACAGTGAGAATGAGGAGGATG | 163 |
Reverse: ACCAGTTCGCTAAGCATGTCCAC | ||
LOC691141 | Forward: AGGCGGTCCAGCTCCTACAAG | 121 |
Reverse: ACACCATACTGACAGCGGAAGTTG | ||
Pdk4 | Forward: GGCCACGGTCGAGCATCAAG | 168 |
Reverse: GGCGTTGGAGCAGTGGAGTATG | ||
Slco2a1 | Forward: ATCCTATTCGCCATCGCTGTGTTC | 94 |
Reverse: TGTCCACTCTGCCGTAGTCCAC | ||
Fgf21 | Forward: TCTCCTGCTGCCTGTCTTCCTG | 129 |
Reverse: TCGGTGTCCTGGTCGTCATCTG | ||
Zbtb16 | Forward: CACCAGCAAGATGTTTGAGATC | 239 |
Reverse: CTGTGTCATTGTCATCAGATGC | ||
Slc10a6 | Forward: TCCTTCTCTGCTGAGTACCTGGTC | 102 |
Reverse: TGCCTGATATGCTGCGACAATGAG | ||
102552154 | Forward: ATCGCTAGTTATGCAGGCATGGAG | 124 |
Reverse: CAGCCAATGAGTTGCACTTGAACC | ||
108351753 | Forward: CAGTGGTTGTGAGCTGTCTGAGG | 148 |
Reverse: ACGCAAGCATGGTAGGTGTTGG | ||
102546487 | Forward: AGCCTCAGCCTTAAGCATGTGTTC | 85 |
Reverse: CCTTGTCTCCTCGGTTGGATGTG | ||
108349645 | Forward: AGGAGCACGGAGATAGCAACTGG | 108 |
Reverse: CGGTGAGTACAGTGCTTGCCATAC | ||
108352578 | Forward: CGCGAACACCGGAACTCTTAGATC | 125 |
Reverse: TGCCACTTGTGCTCAGCTTCTTC |