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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Interleukin-1β mediates alterations in mitochondrial fusion/fission proteins and memory impairment induced by amyloid-β oligomers

Fig. 1

IL-1 antagonist alleviates AβO-induced loss of mitochondrial membrane potential and fusion proteins in mature hippocampal cultures. a Representative images of mature hippocampal cultures exposed to AβOs (500 nM) or vehicle for 24 h. Where indicated, primary cultures were pre-treated for 40 min with anakinra (100 μM). The cultures were stained with a selective mitochondrial probe, Mitotracker (100 nM) (red). b, c Representative images of mature hippocampal cultures immunolabeled for MFN-1 (red) and MFN-2 (green). Scale bar: 60 μm. Optical zoom images of selected regions are also presented. Scale bar: 5 μm. d Integrated immunofluorescence of mitotracker (100 nM). Data expressed as means ± SEM from 4 experiments with independent cultures (30 images analyzed per experimental condition per experiment). *p < 0.05, **p < 0.01 compared with vehicle or AβOs; Two-way ANOVA followed by Holm-Šidak post hoc test. e, f Integrated immunoreactivities for MFN-1 and MFN-2. Data are expressed as means ± SEM from 4 to 5 experiments with independent cultures (30 images analyzed per experimental condition per experiment), *p < 0.05 compared with vehicle or AβOs. Two-way ANOVA followed by Holm-Šidak post hoc test

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