Fig. 1.
From: Systemic administration of β-glucan induces immune training in microglia
BG activates microglia without inducing significant cytokine gene expression in vivo. a Acute stimulation study design. Mice received a single challenge of PBS, LPS (1 mg/kg) or BG (20 mg/kg) by i.p. injection. After 3 h, 1 day and 2 days, animals were terminated. b Microglia were FACS-isolated and gene expression levels of Tnf, Il6, Il1β, Ccl2, Ccl3 and Csf1 were detected by qPCR and normalized to Gapdh gene expression levels (n = 4 mice). c Expression levels of BG receptor gene Dectin-1 and LPS receptor gene Tlr4 were determined by qPCR and normalized to Gapdh gene expression levels (n = 4 mice). Statistical significance was determined with a one-way ANOVA followed a Bonferroni correction for multiple comparisons. *, p < 0.05; **, p < 0.01; ***, p < 0.001.