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Fig. 2. | Journal of Neuroinflammation

Fig. 2.

From: Systemic administration of β-glucan induces immune training in microglia

Fig. 2.

BG does not induce morphological changes in cortical microglia. a Representative images of Iba1-staining in the cortex across groups (scale bar: 50 μm). b Violin plots of representative morphometric parameters for cortical microglia across experimental groups. Violin plots represent the kernel densities for each group. Upper, middle and lower hinges of the boxplots represent the first, second (median) and third quantiles, respectively. Whiskers extend to the minimum and maximum value still within the 1.5 x the interquartile range of the first and third quantiles, respectively. Black dots represent outliers. The significance was determined by Wald chi-square test for linear mixed models. *, p < 0.05; **, p < 0.01; ***, p < 0.001. c Sholl analysis of cortical microglia at 3 h, 1 day and 2 days after LPS or BG injection. PBS-injected mice microglia served as the controls. The vertical lines represent +/- standard deviations. d PCA plot depicts all individual cells selected in cortex on principal component plane. The x and y axes represent the first and second principal components (PC1 and PC2), respectively. e Hierarchical clustering on principal components resulted in 6 cell clusters (I-VI). The dashed line indicates the cut off for 6 clusters. f Representative cells from each cluster are shown. g Cluster distribution analysis of cortical microglia at 3 h, 1 day and 2 days after LPS or BG injection. Animals with PBS injection were used as control. The number of microglia selected in the different groups: PBS: 80 cells; LPS-3h: 67 cells; LPS-1d: 63 cells; LPS-2d: 75 cells; BG-3h: 90 cells; BG-1d: 84 cells; BG-2d: 80 cells (n = 3 mice for each experimental group).

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