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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Senescent accelerated prone 8 (SAMP8) mice as a model of age dependent neuroinflammation

Fig. 2

Morphology analysis by Sholl analysis. (a) (i) Representative images of hippocampus Iba1+ (green) regions used for analysis. so, stratum oriens; sp, stratum pyramidale; sr, stratum radiale. (ii) Detailed capture of cell morphology of an Iba1+ cell (red) maximum projection. (iii) Binary image of the maximum projection of the detailed image. (iv) Cartoon showing the radius of the longest extension that corresponds to the radius of the largest concentric circle and furthest from the soma (ending radius or maximum radius) and the radius that would cover the soma (starting radius or initial radius). (b) (i) Representative images of brain maps from 2 months and 10 months SAM Hpp areas using a ×40 objective and ×1.7 digital zoom. Insets show ×63 detailed images of iba1+ positive binary images for cell morphology. Nuclei are stained with DAPI (blue); brain microglia/macrophages are stained with Iba-1 (red). Scale bars are included in the images. (ii) Sholl analysis results, number of primary branches and branch index from Iba1+ cells from 2 (triangles) and 10 months (circles) old SAM mice Hpp area (n = 3 male). SAMR1 is shown in green and SAMP8 in red. Images were obtained with a Leica SP5 TCS inverted fluorescence confocal microscope, from 30 μm thick cryostat sections

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