Fig. 7

bMyC from brain parenchyma (BP) and m/Ch respond to low doses of systemic LPS. Isolated CD45⁺ cells were isolated form BP and m/Ch preparation as in Fig. 6. (a) BP CD45⁺ (b) m/Ch preparations, from aged (10 months) old SAMR1 (green bars) and SAMP8 (red bars) that were injected with saline (empty bars) or LPS (1 mg/mL) (stripped bars) for 3 h and Il-1β (panel i), Tnf-α (panel ii), Il-6 (panel iii), and Ccl2 (panel iv) mRNA expression was then qRT-PCR as before. Cytokines-specific mRNAs increased significantly 3 h after the injection of LPS. Data in box and whisker plots are presented as dCt (Ct _Gene-Ct _36b4). (mean ±SEM) n = 3 males). Lower values of dCt means higher cytokine transcript expression in the sample. SAMR1 is shown in green and SAMP8 in red. Two months data are represented with triangles and 10 months with circles **p < 0.01 and ***p < 0.001. (ns) means non-significant differences between specified groups, n ≥ 3 males