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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Can quantifying morphology and TMEM119 expression distinguish between microglia and infiltrating macrophages after ischemic stroke and reperfusion in male and female mice?

Fig. 2

Microglia morphologic response to ischemic stroke is not influenced by sex or menopause. a Images of IBA1-positive microglia (× 40 objective) in the cortex of mice after sham and stroke procedure. Three cortical ipsilateral regions in proximity to the infarcted tissue were included in the analysis as depicted in b. Summary data of skeleton analysis show that both the number of microglia process endpoints/cell (c) and summed process length/cell (d) decrease in proximity to the brain injury but with no sex differences. Fractal analysis of individual cells shows that fractal dimension (e) and density (f) are, like skeleton analysis, decreasing in proximity to the brain injury and with sex differences noted between the male and postmenopause group in the proximal region. On the other hand, span ratio (g) was consistent among most regions and sex groups but was dramatically increased in the proximal region of all sex groups. Post hoc analysis is shown in the figure (*p < 0.05, **p < 0.01, ***p < 0.001, and #p < 0.0001 vs. sham region; sex differences are noted by the bracket: *p < 0.05 and **p < 0.01). Male, n = 6; postmenopause female, n = 6; premenopause female, n = 6 for all regions. Scale bar = 20 μm

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