Fig. 4

Gestational sepsis downregulates synaptophysin and PSD95 expression, and induces immune activation in neonates’ brains at P8. a–d Western Blot was performed to detect synaptophysin and β-tubulin in the hippocampus (a), neocortex (b), frontal cortex (c), and cerebellum (d). Each bar is the mean +/− SEM from 4 to 6 animals. e–h Western blot was performed to detect PSD95 and β-tubulin in the hippocampus (e), neocortex (f), frontal cortex (g), and cerebellum (h). Each bar is the mean +/− SEM from 3 to 4 animals. (I – L) The immune response at P8 was evaluated through TNF-α, IL-1β, and IL-6 levels analyzed by ELISA in the hippocampus (i), neocortex (j), frontal cortex (k), and cerebellum (l). Each bar is the mean +/− SEM from 3 animals from different litter each one. *p < 0.05 compared saline to sepsis. NS not significant