Fig. 2
Epitope analysis of GluN1356-385 ABs and their effects in vitro. a ABs were applied to HEK293 cells transfected with wild-type GluN1 or a construct with mutated amino acid 368 (N368Q). b, e Hippocampal neurons were immunostained for surface GluN1and PSD95 after treatment for 24 h with patients’, GluN1 ABs, or control ABs and imaged with confocal microscopy. c, d Western blot analysis of GluN1 on membrane fraction and total cells. f Electrophysiological effects of patients’ AB and GluN1356–385 AB on tetanus-induced LTP on brain slices (f, left) (n = 5). Example traces of fEPSPs recorded before (black) and after (red) TBS (f, left, top). Normalized slope of field excitatory postsynaptic potentials in hippocampal slices after ABs treatment (f, right). g Effects of patients’ AB and GluN1356–385 AB on NMDAR-mediated calcium influx (n = 5). *P < 0.05; **P < 0.01