Fig. 3
Localization of GluN1356-385 ABs and their effects in vivo. a The experimental design for active immunization of mice at different time points. b CSF and serum AB titers of mice during 12 weeks of immunization (n = 3). c Immunostaining of mice IgG in brain slices of mice immunized with GluN1356–385 peptides. d Western blot analysis of GluN1 on membrane fraction and total cell lysates from the hippocampi of immunized mice (n = 5). e Normalized slope of fEPSP in a hippocampal slice of immunized mice (right). Example traces of fEPSPs recorded before (black) and after (red) TBS (left). *P < 0.05