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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Exosomal miR-409-3p secreted from activated mast cells promotes microglial migration, activation and neuroinflammation by targeting Nr4a2 to activate the NF-κB pathway

Fig. 1

Exosomes secreted from activated mast cells promoted microglial migration, activation and neuroinflammation. a Exosomes were extracted from the cell culture supernatants of murine P815 cells (Control exo) or LPS-P815 cells (LPS exo). The shapes and size distributions of these exosomes were observed through TEM and NTA. Scale bar 100 nm. b Representative exosomal markers were detected by Western blot. c Fluorescence microscopy showed the uptake of PKH67-labelled exosomes by murine BV-2 cells. Scale bar 50 μm. d Murine BV-2 microglial migration was evaluated through Transwell assays. Scale bar 10 μm. E, Immunofluorescence staining for Iba1 and CD68 was used to evaluate microglial activation. Scale bar 50 μm. F-I, CD86, IL-1β and inflammatory cytokines (IL-6 and TNF-α) were detected via qRT-PCR and ELISA. Cell culture experiments were performed in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001

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